PCR is a powerful and versatile tool of cloning and recombinant DNA technology, yet it is relatively simple in principle and easy for researchers to perform. Cloned PCR-amplified fragments are commonly used for sequencing, transcription, and expression of genes of interest, among other research goals. To clone a sequence of interest using PCR, the desired fragment is amplified from a DNA source and inserted into a prepared vector; therefore, accurate replication of the target sequence is a critical step of the process. PCR cloning technologies range from direct vector insertions such as TA and blunt-end cloning to more specialized applications such as multifragment assembly and mutagenesis.
In this webinar we'll discuss:
- Critical considerations for insert preparation
- Fundamentals of various PCR cloning techniques
- Tips for successful PCR cloning
About the presenter
Thant Zhu, PhD
Market Development Manager, Molecular Biology
Thermo Fisher Scientific
Dr. Zhu studied microbiology for his undergraduate degree, then received his PhD in biochemistry from the University of Illinois at Urbana-Champaign and did postdoctoral cancer research at the University of Michigan. After working as a researcher for over 10 years, he became a technical applications scientist in molecular biology at Thermo Fisher Scientific. Thant is now responsible for the development of technical educational content for the molecular biology portfolio.
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