High-Throughput Methods Evaluation: Impurities Determination During Upstream and Downstream In-Process Development

Zimmerman E, Wang L, Zhang L, et al. (2016)
BioProcess International 14(2):24–32.

In this article, scientists from Boehringer Ingelheim compare three new methods as alternatives to ELISA-based detection of host-cell proteins (HCPs) and leached protein A in bioprocess solutions.

Read about their findings when using the SP-PLA (solid phase-proximity ligation assay) method with the Applied Biosystems™ ProteinSEQ™ Protein Quantitation system:

• Improved overall assay performance with broad dynamic range
  (4 log versus 2 log range with ELISA)
• Increased assay precision and reduced non-specific/cross-reactive antibody binding
• Greater assay sensitivity for protein A
• Approximately two-fold increase in throughput relative to ELISA

Complete and submit the form to receive your free download of this article.