Cloning is an important part of many molecular biology workflows. There are many different technologies available to scientists, which have evolved over time. Ligation-independent cloning (LIC) is one of the less frequently used methods, mainly because researchers are unfamiliar with its inherent benefits. After this webinar you will know the basic principles and variations of the LIC technique, and its advantages over traditional cloning methods, and you will be able to determine key points for optimizing LIC performance. Additionally, you will learn how LIC can be useful for downstream applications such as protein expression in E. coli.
This webinar is ideal for both new and experienced molecular biologists.
Topics covered
- The molecular cloning workflow and available technologies
- LIC overview, including:
- Benefits
- How it works
- Workflow
- Comparison to restriction digest–based cloning methods
- Optimization and tips for:
- PCR primer design and amplification
- Purification
- Cloning reaction setup and transformation
- Troubleshooting tips for:
- Low efficiency/high background
- Cloned sequence errors
Please check the webinar times below for your region.
About the presenter
John Poland, BA
Staff Scientist, Molecular Biology
Thermo Fisher Scientific
John Poland is a staff scientist in the molecular biology group at Thermo Fisher Scientific. John received his bachelor’s degree from the University of California, Santa Cruz, and spent over 14 years working in industrial biotechnology prior to joining Thermo Fisher Scientific in November 2015. John has expertise in molecular cloning, protein expression, protein engineering, and enzymology.
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