Free webinar—TCR and BCR sequencing solutions: applications to clinical research projects
The application of next-generation sequencing to interrogate immune repertoires and methods in which these highly complex datasets can be mined to examine immune regulation and/or identify potential biomarkers has evolved considerably over the years. With advances in sequencing technologies and development of comprehensive, intuitive analysis tools, NGS-based repertoire analysis has become more accessible to the broader research community. Thermo Fisher Scientific’s streamlined and user-friendly TCR and BCR sequencing laboratory and analysis pipelines enable just about anyone to incorporate this powerful tool into their research program.
In this discussion, we present examples of how repertoire analysis can be used to answer different types of research questions. Summaries of different studies leveraging TCR and BCR sequencing in human disease research to better understand mechanisms involved in inflammatory diseases are presented. Methods of experimental design, sequencing, and analysis are introduced for each, with examples of figures that can be generated with the user-friendly analysis portal.
- The importance of biomarker development and discovery in inflammatory diseases, including those caused by infectious agents like SARS-CoV-2
- Molecular applications in immune repertoire sequencing and immune response
- Basic methods of analysis
Brian P. O'Connor, PhD
Associate Professor and Scientific Director of Genomics
National Jewish Health Center for Genes, Environment, and Health
Dr. O'Connor's work focuses on understanding how epigenetic mechanisms regulate the decision processes governing immune cell activity in the context of disease. The immune system is comprised of multiple types of autonomous cells that must work together to influence the outcome of disease. The identity and function of the autonomous immune cells are influenced by microenvironmental stimuli, which in turn affect the epigenome. Currently, Dr. O'Connor's lab examines the cross talk between environmental stimuli (such as diet or inflammation), the immune system, and disease (such as asthma).
Dr. O'Connor received his PhD in immunology and has been trained in organismal, cellular, and molecular immunology. His post-doctoral training was in immune epigenetics and molecular biology.
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