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In this video, you'll get a step‑by‑step overview on how to use Thermo Fisher Scientific's digitally compatible, GMP‑compliant manufacturing platform to produce CAR T cells.

You'll learn about:

• Preparation of media and reagents used for cell isolation and activation
• Set up of consumable and instrument for cell processing using the Gibco CTS Rotea Counterflow Centrifugation System
• Isolation and activation of the cells using the Gibco CTS DynaMag Magnet and CTS Rotea System
• Transfer of the target cells to the bioreactor using the Thermo Scientific HyPerforma G3Lab controller for culture and lentiviral infection
• Bead removal, cell wash and concentrate and cryopreservation of the final CAR T cell product

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