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Protein isolation & purification

Due to the heterogeneity of proteins, there is no one method or reagent that is optimal for general protein isolation and purification. The first step in protein analysis is cellular extraction. Following lysis, and depending on the next step in the workflow, the protein extract may require further clean-up, using techniques such as dialysis, desalting, or concentration, or the protein of interest may be enriched or more fully separated, using immunoprecipitation or affinity purification.
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Subcellular Fractionation • Protease & Phoephatase Inhibitors
Dialysis • Deasalting • Concentration • Purification
Immunoprecipitation