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Western blotting

The term "blotting" refers to the transfer of biological samples from a gel to a membrane and their subsequent detection on the surface of the membrane. Western blotting was introduced by Towbin, et al. in 1979 and is now a routine technique for protein analysis. The specificity of the antibody-antigen interaction enables a target protein to be identified in the midst of a complex protein mixture. Western blotting can produce qualitative and semiquantitative data about that protein.
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Transfer System & Buffers • Primary & Secondary Antibodies
Blocking Buffers • Substrates • Imaging Systems
Stripping Buffers