Crosslinker Selection Tool

Conjugation

to

Alkyne

Amines (e.g., lysine)

Azide

Carbohydrates (Oxidized sugars)

Carboxyls (e.g., C-terminus)

NA (Metabolic labeling)

Nonselective (Photoreactive)

Phosphine

Sulfhydryls (e.g., cysteine)

Alkyne

Amines (e.g., lysine)

Azide

Carbohydrates (Oxidized sugars)

Carboxyls (e.g., C-terminus)

NA (Metabolic labeling)

Nonselective (Photoreactive)

Phosphine

Sulfhydryls (e.g., cysteine)

Cleavability

Any

Not cleavable

By Thiols

By Base

By Periodate

By Hydroxylamine

By CID using MS

Water soluble*

Any

Yes

No

Pegylated

Any

Yes

No

Membrane permeable

Any

Yes

No

Spacer arm length**

Any

Short

Medium

Long

Packaging^†

Any

Premium grade

Single use

Standard