Gibco cell culture products – Driving innovation for 60 years

We go beyond basic formulations to provide optimized and innovative cell culture solutions in support of your efforts to achieve consistently reproducible results. Gibco media, sera, supplements, and cell culture reagents are designed to deliver reproducibility and performance for results you can count on. Regardless of whether you are performing cell culture in a research lab or production facility, need a special or defined formulation, or are growing cell lines, primary cells, or stem cells, Gibco products offer a reliable solution.

Gibco cell culture products – Driving innovation for 60 years

We go beyond basic formulations to provide optimized and innovative cell culture solutions in support of your efforts to achieve consistently reproducible results. Gibco media, sera, supplements, and cell culture reagents are designed to deliver reproducibility and performance for results you can count on. Regardless of whether you are performing cell culture in a research lab or production facility, need a special or defined formulation, or are growing cell lines, primary cells, or stem cells, Gibco products offer a reliable solution.

Est. 2008

The Gibco Media bottle

This iconic bottle was designed by listening to you. It provides better ergonomic features, ease of use, and reduced chance of contamination. The compact design of the Gibco media bottle allows for easier handling and storage, and also reduced packaging.

Discover more Gibco bottle benefits
Est. 2008

The Gibco Media bottle

This iconic bottle was designed by listening to you. It provides better ergonomic features, ease of use, and reduced chance of contamination. The compact design of the Gibco media bottle allows for easier handling and storage, and also reduced packaging.

Discover more Gibco bottle benefits
Cell Culture Media & Reagents
Cell Culture Fetal Bovine Serum
Pluripotent Stem Cell Culture
Neuronal Cell Culture
Protein Expression Systems
Cell Therapy Systems
Time-tested and trusted, Gibco cell culture media and reagents are designed to support the growth and maintenance of a variety of mammalian, insect and microbial cells and cell lines. Explore Gibco media for research use at thermofisher.com/media and for production use at thermofisher.com/gibcobioprocessing
2021

Human Plasma-like Medium

A new formulation designed to resemble the natural cellular environment found in the body, mimicking the metabolic profile of human plasma. HPLM provides researchers the ability to study the impact of physiologically relevant cell media on their specific applications.

Learn more
2019

BenchStable Media

Engineered for flexibility and convenience to enable storage at room temperature. Available in the most commonly used basal media formulations: DMEM, DMEM/F-12, MEM, and RPMI 1640. BenchStable media have been optimized for routine cell culture, maintaining expected morphology and function of many common cell lines.

Learn more
2012

Freedom kits for cloning and expression

Gibco Freedom kits contain all the components for beginning-to-end cloning and expression, within a short development time. Each kit includes cGMP compliant products, with no need to source components or develop vectors, allowing you to focus on quickly moving your workflow from cell line to process development. Our Freedom kits also offer commercial licensing that’s simple, flexible, and without royalties.

Learn more
2003

TrypLE Cell Dissociation Reagents

The superior replacement for trypsin. TrypLE reagents are gentle on cells, protecting your cell’s surface proteins. Its exceptional purity increases specificity and reduces damage to cells that can be caused by other enzymes present in some trypsin extracts.

Learn more
2002

Advanced Media

Consistent cell growth, less serum, lower cost. Enriched with normal-serum constituents, Gibco Advanced media can be used in combination with FBS, and feature formulations that support common cell lines (e.g. DMEM, DMEM/F-12, MEM, RPMI 1640). Experience fewer lot-to-lot changes of serum, meaning less variability, along with reduced cost due to lower serum usage and fewer lots tested.

Learn more
2002

Advanced Granulation Technology format (AGT)

The AGT medium format was developed to help overcome multi-step dry powder media preparation and filtration challenges and to decrease handling safety risks. AGT granules dissolve rapidly to decrease media preparation time. The AGT dry media format is designed to provide increased consistency and productivity across all stages of production from development to commercial manufacturing.

Learn more
1994

GlutaMAX Supplement

More stable than L-glutamine for keeping your cells healthier longer. Gibco GlutaMAX Supplement is a stabilized form of L-glutamine and features superior stability to help prevent degradation during storage or in the presence of cells, which happens with L-glutamine. Available as a stand-alone supplement or as a component in Gibco media products.

Learn more
2021

Human Plasma-like Medium

A new formulation designed to resemble the natural cellular environment found in the body, mimicking the metabolic profile of human plasma. HPLM provides researchers the ability to study the impact of physiologically relevant cell media on their specific applications.

Learn more
2019

BenchStable Media

Engineered for flexibility and convenience to enable storage at room temperature. Available in the most commonly used basal media formulations: DMEM, DMEM/F-12, MEM, and RPMI 1640. BenchStable media have been optimized for routine cell culture, maintaining expected morphology and function of many common cell lines.

Learn more
2012

Freedom kits for cloning and expression

Gibco Freedom kits contain all the components for beginning-to-end cloning and expression, within a short development time. Each kit includes cGMP compliant products, with no need to source components or develop vectors, allowing you to focus on quickly moving your workflow from cell line to process development. Our Freedom kits also offer commercial licensing that’s simple, flexible, and without royalties.

Learn more
2003

TrypLE Cell Dissociation Reagents

The superior replacement for trypsin. TrypLE reagents are gentle on cells, protecting your cell’s surface proteins. Its exceptional purity increases specificity and reduces damage to cells that can be caused by other enzymes present in some trypsin extracts.

Learn more
2002

Advanced Media

Consistent cell growth, less serum, lower cost. Enriched with normal-serum constituents, Gibco Advanced media can be used in combination with FBS, and feature formulations that support common cell lines (e.g. DMEM, DMEM/F-12, MEM, RPMI 1640). Experience fewer lot-to-lot changes of serum, meaning less variability, along with reduced cost due to lower serum usage and fewer lots tested.

Learn more
2002

Advanced Granulation Technology format (AGT)

The AGT medium format was developed to help overcome multi-step dry powder media preparation and filtration challenges and to decrease handling safety risks. AGT granules dissolve rapidly to decrease media preparation time. The AGT dry media format is designed to provide increased consistency and productivity across all stages of production from development to commercial manufacturing.

Learn more
1994

GlutaMAX Supplement

More stable than L-glutamine for keeping your cells healthier longer. Gibco GlutaMAX Supplement is a stabilized form of L-glutamine and features superior stability to help prevent degradation during storage or in the presence of cells, which happens with L-glutamine. Available as a stand-alone supplement or as a component in Gibco media products.

Learn more
Gibco serum has earned the trust of researchers around the world due to consistent quality, commitment to innovation, and award-winning technical support. Discover all FBS solutions at thermofisher.com/fbs ›
2016

One Shot FBS 50 mL Bottle

One Shot FBS 50 mL bottle was designed specifically for FBS use to minimize contamination and save time. This unique little bottle makes it possible to eliminate the need to aliquot and reduce plastic waste by 33%. Discover why so many researchers love the benefits that come with this One Shot bottle

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2014

Origin and traceability reassurance

Origin and traceability reassurance like no other: Not only have we’ve been ISIA Traceability Certified since 2014, but we were the first serum supplier to provide fetal bovine serum (FBS) fingerprinting technology powered by Oritain to provide empirical and statistical certainty over the origin and authenticity. We consider it imperative that our customers know exactly what they ‘re getting and have confidence that our products will enable the results they demand.

Learn More
2012

Gibco iMATCH Sera Lot Matching Tool

The powerful Gibco iMATCH Sera Lot Matching Tool has changed the way researchers make serum choices. Researchers can spend more time on discovery and less time on tasks that don’t add value, like testing serum. iMatch can provide consistency and convenience for researchers, with lot matching negating the need and time to test and establish large reserves.

Learn More
2016

One Shot FBS 50 mL Bottle

One Shot FBS 50 mL bottle was designed specifically for FBS use to minimize contamination and save time. This unique little bottle makes it possible to eliminate the need to aliquot and reduce plastic waste by 33%. Discover why so many researchers love the benefits that come with this One Shot bottle

Learn more
2014

Origin and traceability reassurance

Origin and traceability reassurance like no other: Not only have we’ve been ISIA Traceability Certified since 2014, but we were the first serum supplier to provide fetal bovine serum (FBS) fingerprinting technology powered by Oritain to provide empirical and statistical certainty over the origin and authenticity. We consider it imperative that our customers know exactly what they ‘re getting and have confidence that our products will enable the results they demand.

Learn more
2012

Gibco iMATCH Sera Lot Matching Tool

The powerful Gibco iMATCH Sera Lot Matching Tool has changed the way researchers make serum choices. Researchers can spend more time on discovery and less time on tasks that don’t add value, like testing serum. iMatch can provide consistency and convenience for researchers, with lot matching negating the need and time to test and establish large reserves.

Learn more
Thermo Fisher has been developing products to meet the needs of stem cell researchers for decades. From the early days of serum, which was highly variable, we have innovated our PSC media offering as the field has advanced. Discover all stem cell culture solutions at thermofisher.com/stemcellculture ›
2021

StemScale PSC Suspension Medium

A new dimension to PSC culture; A scalable, easy-to-use medium designed for PSC suspension culture that delivers 5–10-fold expansion per passage. Researchers can confidently scale up for applications like drug discovery, cell therapy development, and 3D stem cell biology.

Learn More
2017

StemFlex Medium

Designed for today’s applications; 2-fold faster recovery following gene editing, 5-fold improvement in clonal expansion following single-cell passaging, and maximal workflow flexibility.

Learn More
2012

Essential 8 family

Fully defined, xeno free formulations containing only the 8 essential components needed to grow feeder-free. Versions available as CTS and for weekend-free feeding.

Learn More
1998

KnockOut Serum Replacement

The most published reagent for feeder-dependent mouse ESC cultures as a direct replacement for fetal bovine serum.

Learn More
2021

StemScale PSC Suspension Medium

A new dimension to PSC culture; A scalable, easy-to-use medium designed for PSC suspension culture that delivers 5–10-fold expansion per passage. Researchers can confidently scale up for applications like drug discovery, cell therapy development, and 3D stem cell biology.

Learn more
2017

StemFlex Medium

Designed for today’s applications; 2-fold faster recovery following gene editing, 5-fold improvement in clonal expansion following single-cell passaging, and maximal workflow flexibility.

Learn more
2012

Essential 8 family

Fully defined, xeno free formulations containing only the 8 essential components needed to grow feeder-free. Versions available as CTS and for weekend-free feeding.

Learn more
1998

KnockOut Serum Replacement

The most published reagent for feeder-dependent mouse ESC cultures as a direct replacement for fetal bovine serum.

Learn more
Our Gibco neuronal and neural stem cell culture media and supplements are the most widely used for neural cell culture. All of our products have been developed to work together for optimal performance and to create, complete neuronal, glial, and neural stem cell culture systems. Discover all neurobiology research solutions at thermofisher.com/neuro ›
2019

B-27 Plus Supplement

The most-referenced neuronal cell culture supplement just got better. To further meet the growing needs of researchers, an optimized formulation of the classic B-27 Supplement was developed in 2018 for improved long-term neuronal survival, functionality and maturation of primary rodent neurons and stem cell-derived neurons.

Learn More
1992

B-27 Supplement

The first commercially available, serum-free supplement for neural cell culture, Gibco B-27 Supplement (50X) has set the standard in neuronal cell culture reagents. Originally designed for culturing primary neurons, the classic B-27 Supplement and nutritive variants have been widely adapted and successfully used in many other applications and cell types, including growth and maintenance of neural stem cells and PSC-derived neurons, 3D cell culture and disease modeling.

Learn More
1992

Neurobasal Media

Provides long-term maintenance of the normal phenotype and growth of neuronal cells, maintaining pure populations of neuronal cells without the need for an astrocyte feeder layer. Gibco Neurobasal Plus Medium is the next-generation version of Neurobasal, designed to enable the highest in vitro survival of primary rodent and human stem cell–derived neurons when used with B-27 Plus Supplement.

Learn More
2019

B-27 Plus Supplement

The most-referenced neuronal cell culture supplement just got better. To further meet the growing needs of researchers, an optimized formulation of the classic B-27 Supplement was developed in 2018 for improved long-term neuronal survival, functionality and maturation of primary rodent neurons and stem cell-derived neurons.

Learn more
1992

B-27 Supplement

The first commercially available, serum-free supplement for neural cell culture, Gibco B-27 Supplement (50X) has set the standard in neuronal cell culture reagents. Originally designed for culturing primary neurons, the classic B-27 Supplement and nutritive variants have been widely adapted and successfully used in many other applications and cell types, including growth and maintenance of neural stem cells and PSC-derived neurons, 3D cell culture and disease modeling.

Learn more
1992

Neurobasal Media

Provides long-term maintenance of the normal phenotype and growth of neuronal cells, maintaining pure populations of neuronal cells without the need for an astrocyte feeder layer. Gibco Neurobasal Plus Medium is the next-generation version of Neurobasal, designed to enable the highest in vitro survival of primary rodent and human stem cell–derived neurons when used with B-27 Plus Supplement.

Learn more
Using the right protein expression system for your specific application is critical to success. We offer a wide selection of superior mammalian, insect, and other host systems to suit your research needs. Find your system at thermofisher.com/proteinexpression ›
2017

ExpiSf Expression System

The first-ever chemically defined baculovirus expression system that can generate three times more protein compared to existing insect expression platforms. Get greater consistency across multiple expression runs, all in less time.

Learn More
2015

ExpiCHO Expression System

Maintain protein quality and function from research to bioproduction. This completely optimized system provides the highest yields possible in a transient system (up to 3 g/L). That means you can start your research work in CHO cells and stay in CHO cells through discovery.

Learn More
2012

Expi293 Expression System

Trust the industry standard for rapid, high-yield transient HEK 293 expression. The components of this rapid, high-yield protein production system work together to allow access to recombinant 293-derived proteins in just five to seven days, with a 2–10-fold increase in protein yields.

Learn More
2017

ExpiSf Expression System

The first-ever chemically defined baculovirus expression system that can generate three times more protein compared to existing insect expression platforms. Get greater consistency across multiple expression runs, all in less time.

Learn more
2015

ExpiCHO Expression System

Maintain protein quality and function from research to bioproduction. This completely optimized system provides the highest yields possible in a transient system (up to 3 g/L). That means you can start your research work in CHO cells and stay in CHO cells through discovery.

Learn more
2012

Expi293 Expression System

Trust the industry standard for rapid, high-yield transient HEK 293 expression. The components of this rapid, high-yield protein production system work together to allow access to recombinant 293-derived proteins in just five to seven days, with a 2–10-fold increase in protein yields.

Learn more
Supporting your move from basic cell therapy research to the clinic, Cell Therapy Systems (CTS) products provide a proven choice for cell-based immunotherapy research, clinical stem cell therapy and manufacturing. In addition to providing serum-free, xeno-free and animal origin-free reagent formulations and innovative cell processing instruments our GMP-grade CTS products have cell and gene therapy specific intended use statements, extensive safety testing and proactive regulatory documentation. Discover all clinical cell therapy manufacturing solutions at thermofisher.com/CTS ›
2022

CTS Xenon Electroporation System

The CTS Xenon Electroporation System, a large volume electroporation system designed for cell therapy development and manufacturing, offers reliably high transfection performance in 1 or 5-25 mL with exceptional cell viability and recovery. The intuitive, programmable interface, process flexibility, sterile single-use consumables, and available software upgrade that helps enable 21 CFR Part 11 compliance allow the system to seamlessly scale with your cell therapy workflow from process development through clinical manufacturing.

Learn More
2021

CTS NK-Xpander Medium

Designed to proliferate a high yield of functional human natural killer (NK) cells with or without the use of feeder cells, CTS NK-Xpander Medium was developed to address the needs of translational and clinical allogeneic NK cell therapy researchers.

Learn More
2020

CTS Rotea Counterflow Centrifugation System

The multipurpose Gibco CTS Rotea Counterflow Centrifugation System offers exceptional flexibility in a cell therapy development and manufacturing system. This versatile system applies the proven counterflow centrifugation method to a broad range of cell processing applications, such as CAR-T therapy, stem cell therapy, and PBMC isolation.

Learn More
2020

CTS Bagged Media

Many of our Gibco CTS reagents are now offered in a BioProcess Container format suitable for a closed system workflow to help you close and scale your process, reducing the risk of contamination or human error and increasing your manufacturing efficiency. This can help boost the likelihood of clinical and commercial manufacturing success while also reducing the need for costly Class A clean room use.

Learn More
2019

CTS LV-MAX Lentiviral Production System

A complete, optimized serum-free system designed to support high-titer lentiviral production in a variety of suspension culture vessels, enabling you to scale up or down based on your needs. This cost-effective and scalable lentiviral manufacturing platform can support high throughput early in discovery or seamless clinical scale-up, all while maintaining high yield.

Learn More
2010

CTS AIM V Medium

Gibco CTS AIM V SFM is the first commercially available, defined, serum-free medium (SFM) for the proliferation and/or manipulation of T cells and dendritic cells manufactured in compliance with cGMP.

Learn More
2010

CTS Dynabeads CD3/CD28

Gibco CTS Dynabeads magnetic beads are a trusted technology for the isolation, activation, and expansion of T cells ex vivo. Dynabeads CD3/CD28 can be used for the isolation, activation, and/or expansion of polyclonal T cells, antigen-specific T cells, gene-modified T cells, and other T cell subtypes and will deliver 100- to 1,000-fold expansion in 9–14 days.

Learn More
2010

CTS OpTmizer Pro SFM

Gibco CTS OpTmizer Pro SFM is the first medium developed for the growth and expansion of human T lymphocytes (e.g., CD4+, CD8+, polyclonal, or antigen-specific T cells) used in allogeneic cell therapies. This serum-free and xeno-free medium improves central memory phenotype and cell growth, resulting a larger population of central memory cells in a shorter period of time.

Learn More
2022

CTS Xenon Electroporation System

The CTS Xenon Electroporation System, a large volume electroporation system designed for cell therapy development and manufacturing, offers reliably high transfection performance in 1 or 5-25 mL with exceptional cell viability and recovery. The intuitive, programmable interface, process flexibility, sterile single-use consumables, and available software upgrade that helps enable 21 CFR Part 11 compliance allow the system to seamlessly scale with your cell therapy workflow from process development through clinical manufacturing.

Learn more
2021

CTS NK-Xpander Medium

Designed to proliferate a high yield of functional human natural killer (NK) cells with or without the use of feeder cells, CTS NK-Xpander Medium was developed to address the needs of translational and clinical allogeneic NK cell therapy researchers.

Learn more
2020

CTS Rotea Counterflow Centrifugation System

The multipurpose Gibco CTS Rotea Counterflow Centrifugation System offers exceptional flexibility in a cell therapy development and manufacturing system. This versatile system applies the proven counterflow centrifugation method to a broad range of cell processing applications, such as CAR-T therapy, stem cell therapy, and PBMC isolation.

Learn more
2020

CTS Bagged Media

Many of our Gibco CTS reagents are now offered in a BioProcess Container format suitable for a closed system workflow to help you close and scale your process, reducing the risk of contamination or human error and increasing your manufacturing efficiency. This can help boost the likelihood of clinical and commercial manufacturing success while also reducing the need for costly Class A clean room use.

Learn more
2019

CTS LV-MAX Lentiviral Production System

A complete, optimized serum-free system designed to support high-titer lentiviral production in a variety of suspension culture vessels, enabling you to scale up or down based on your needs. This cost-effective and scalable lentiviral manufacturing platform can support high throughput early in discovery or seamless clinical scale-up, all while maintaining high yield.

Learn more
2010

CTS AIM V Medium

Gibco CTS AIM V SFM is the first commercially available, defined, serum-free medium (SFM) for the proliferation and/or manipulation of T cells and dendritic cells manufactured in compliance with cGMP.

Learn more
2010

CTS Dynabeads CD3/CD28

Gibco CTS Dynabeads magnetic beads are a trusted technology for the isolation, activation, and expansion of T cells ex vivo. Dynabeads CD3/CD28 can be used for the isolation, activation, and/or expansion of polyclonal T cells, antigen-specific T cells, gene-modified T cells, and other T cell subtypes and will deliver 100- to 1,000-fold expansion in 9–14 days.

Learn more
2010

CTS OpTmizer Pro SFM

Gibco CTS OpTmizer Pro SFM is the first medium developed for the growth and expansion of human T lymphocytes (e.g., CD4+, CD8+, polyclonal, or antigen-specific T cells) used in allogeneic cell therapies. This serum-free and xeno-free medium improves central memory phenotype and cell growth, resulting a larger population of central memory cells in a shorter period of time.

Learn more

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About Thermo Fisher Scientific

Thermo Fisher Scientific is the world leader in serving science, with revenues of more than $24 billion and approximately 70,000 employees globally. Our mission is to enable our customers to make the world healthier, cleaner and safer. We help our customers accelerate life sciences research, solve complex analytical challenges, improve patient diagnostics, deliver medicines to market and increase laboratory productivity. Through our premier brands – Thermo Scientific, Applied Biosystems, Invitrogen, Fisher Scientific and Unity Lab Services – we offer an unmatched combination of innovative technologies, purchasing convenience and comprehensive services.

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