b'AntibodiesWe are continually updating our Invitrogen primary antibodyDue to its localization, MOG is a potential target of immune-mediated portfolio with advanced verified products for use in flow cytometrydemyelination and has been regarded as a putative autoantigen in (FC), immunohistochemistry (IHC), immunofluorescence (IF),individuals with inflammatory demyelinating diseases. immunocytochemistry (ICC), western blotting (WB), enzyme-linked immunosorbent assays (ELISAs), and other applications.Antibodies against MOG act as specific biomarkers for a subset of acquired demyelinating syndromes known as myelin-oligodendrocyte Myelin-oligodendrocyte glycoproteinglycoprotein antibody-associated diseases (MOGAD). These antibodies are detected in individuals with optic neuritis, myelitis, opticomyelitis, antibody for neuro-inflammatoryand acute or multiphasic disseminated encephalomyelitis disease research (ADEM/MDEM) but not in individuals with multiple sclerosis (MS). MOG antibodies serve as an important marker for oligodendrocyte maturation Myelin-oligodendrocyte glycoprotein (MOG), a member of theattributable to its expression in the later stages of myelinogenesis. immunoglobulin (Ig) superfamily, is a minor myelin protein expressedAntibodies that are functionally verified for specificity can help provide on the outer surface of myelin sheaths and oligodendrocytes. It isconfidence in disease research (Figure 2).expressed primarily within the brain, spinal cord, and the optic nerves.Mouse brain Mouse cerebellum Mouse heart Mouse liverRat brain Rat cerebellum Rat heart Rat liver26016011080 Figure 2. Specificity of the MOG antibody shown by relative expression in a western blot where MOG is detected distinctively in brain and cerebellum tissue extracts 60 and not in heart or liver. Western blot analysis was performed using the Invitrogen MOG Recombinant Rabbit Monoclonal Antibody (JM23-06) (Cat. No. MA5-32857), and 50 a 27 kDa band corresponding to MOG was observed specifically in brain and cerebellum tissues. Extracts of the indicated tissue (30 g lysate) were electrophoresed on an 40 Invitrogen NuPAGE 10% Bis-Tris Mini Protein Gel (Cat. No. NP0301BOX). Resolved MOG proteins were then transferred onto an Invitrogen iBlot 2 Transfer Stack, nitrocellulose 30 ~ 27 kDa membrane (Cat. No. IB23001), using the Invitrogen iBlot 2 Gel Transfer Device (Cat. No. IB21001). The blot was probed with the primary antibody (1:5,000 dilution) 20 and detected by chemiluminescence with the Invitrogen Goat AntiRabbit IgG (H+L), Superclonal Recombinant Secondary Antibody, HRP (Cat. No. A27036, 1:4,000 dilution), using the Invitrogen iBright FL1500 Imaging System (Cat. No. A44115). 15 Chemiluminescent detection was performed using the Thermo Scientific Pierce ECL Western Blotting Substrate (Cat. No. 32106).103.5HSP70See MOG antibodies and more at thermofisher.com/antibodiesContents Inside the Cell//Issue No. 12 15'