b'PCR primers and DNA oligos for cloning applicationsWell-designed, high-quality primers are essential for successful PCR. In general, the optimal length of PCR primers is 18 to 30 nucleotides. The melting temperatures of primers in a primer set should be between 65C and 75C, and they should be within 5C of each other. Use the free OligoPerfect Primer Designer to design your primers. You can add restriction sites or directional cloning sequences with the click of a button. For cloning applications, we recommend HPLC or PAGE purification of your oligos. Check out the OligoPerfect Primer Designer to get tips for primer design.Video: Tips for primer design ry the OligoPerfect Primer Designer at thermofisher.com/oligoperfectTContents 11'