b'TransformationOnce a DNA fragment is cloned into a vector, it can be used to transform Escherichia coli, which will produce a sufficient quantity of DNA or protein for downstream experiments. The type of competent cell used for transformation will depend on the transformation method, the strain genotype, plasmid characteristics, and the desired application. We offer a variety of Invitrogen competent E. coli strains that are suitable for different applications. If you are using an electroporator for transformation, be sure to select an electrocompetent format.Visit thermofisher.com/compcells-education for technical resources about competent cells.Choose competent cells basedIs this for routine cloning on your application and subcloning?Yes NoRoutine cloning Protein productionIs high transformationYes Noefciency needed?Yes No Protein production Contact technical support thermosher.com/contactOne Shot (DE3 ) BL21 StarHigh transformationStandard efciency High mRNA stability forefciency (cloning) (propagation)increased protein expressionOne ShotOmniMAX2 T1 Subcloning EfciencyDH5BL21(DE3) ROne Shot (5 x 109 cfu/g)(1 x 106 cfu/g) High-level expressionof recombinant proteinsOne Shot TOP10BL21(DE3) pLysE (1 x 109 cfu/g)One Shot One Shot DH10B T1 R Lower basal expressionof T7 promoter genes(1 x 109 cfu/g) BL21 Star (DE3) pLysS MAX EfciencyDH5One Shot (1 x 109 cfu/g)Low basal expression, faster Library EfciencyDH5* induction of T7 promoter genes8(1 x 10cfu/g)One ShotBL21-AIOne Shot Mach1-T1 R Best for expression of(1 x 10 cfu/g) toxic proteins9Electrocompetent cells.* Not for methylated or genomic DNA. Find out more at thermofisher.com/compcells 8 Contents'