b'TOPO cloningInvitrogen TOPO PCR cloning technology improves cloning efficiency, simplifies workflows, saves time, and works with a wide range of PCR insert sizes. TOPO cloning vectors are linearized by topoisomerase I, which also has a ligase function. The enzyme covalently binds to the 3 phosphate group on each end of the vector. This enables the vector to be joined to a PCR insert with compatible ends in 5 minutes with up to 95% efficiency and no additional ligation steps. Quickly find the TOPO cloning kit that best fits your needs, using the TOPO PCR Cloning Selection Tool.Perform PCR with Taq orAdd 1 L of PCRIncubate 5 minutes at Perform transformationFPOproduct to 1 L ofwith provided competent proofreading polymerase TOPO cloning vector room temperature E. coli strainA PCR product A PCR productCACC PCR productTOPOTOPOTOPOcloningvector Watch the TOPO cloning video at thermofisher.com/topo.TOPO PCR cloning is done in three easy steps.Did you know?The Invitrogen TOPO XL-2 Complete PCR Cloning Kit has all the components needed for highly efficient cloning of extra long (113 kb) PCR products.Find out more at thermofisher.com/topoContents 5'