b'ContentsCloningFragment verification and analysisOverview based on insert size and complexity 4Nucleic acid electrophoresis workflow 14 TOPO vectors for PCR cloning 5 E-Gel precast gels15Restriction enzymes 6 CloneWell gels 15Cloning with synthetic DNA 7 Electrophoresis reagents15Transformation8Gibson Assembly cloning 9 Sample preparation for cloningType IIs cloning 9 Purification of PCR products 17 Advanced plasmid DNA purification17PCR and primer design Automated purification solutions18 Designing your primers 11 Choosing a PCR enzyme12 Contents 3'