b'PCR fidelityInvitrogen Platinum SuperFi II DNA Polymerase is our highest-fidelity DNAPlatinum SuperFi II enzymepolymerase. It is ideally suited for cloning and other applications that requireVendor 1 enzymeexceptional sequence fidelity to help you avoid introducing errors into your clone.TVendor 2 enzymePlatinum SuperFi II DNA Polymerase efficiently amplifies sequences up to 20 kb inVendor 3 enzymelength and sequences that are difficult to amplify.Vendor 4 enzymeFind out more at thermofisher.com/superfi Vendor 5 enzymeTaq enzyme0 100 200 300Fidelity compared to Taq enzymePlatinum SuperFi II DNA Polymerase preserves sequence accuracy with an extremely low error rate. A 3.9 kb sequence was amplified by PCR using Platinum SuperFi II DNA Polymerase, Taq polymerase, and DNA polymerases from different vendors. The amplicons were then fragmented with a MuA transposase. Unique molecular identifiers were introduced during fragmentation to individually tag each product. Next-generation sequencing and sequence alignment showed the fidelity of Platinum SuperFi II DNA Polymerase to be over 300 times higher than the fidelity of Taq polymerase and significantly higher than that of enzymes from other vendors. Video: What is high-fidelity PCR? Learn about DNA polymerase fidelity, methods for measuring enzyme fidelity, and the benefits of using a high-fidelity DNA polymerase for PCR.Find out more at thermofisher.com/pcrworkflow 12 Contents'