b'Sample preparationSelecting the right DNA purification kitsComparison of DNA cleanup solutionsDNA cleanupPCR cleanup Sequencingapplication PCR cleanup PCR cleanup PCR cleanup Gel extraction and gel extraction PCR cleanup reaction cleanupPureLink Quick Gel PureLink PCRPureLink Pro 96 PCRPureLink PCR MicroPureLink Quick GelExtraction Kit and PCRChargeSwitch-ProCentri-Sep Spin Kit Purification Kit Purification Kit Kit Extraction Kit Purification Combo Kit PCR Clean-Up Kit ColumnsFormat Silica spin/ 96-well silica plate Silica spin column Silica spin/ Silica spin/ Derivatized spin/ Spin columnvacuum column vacuum column vacuum column vacuum column 10 preps10 preps 50 preps4 plates50 preps100 columnsProduct size (4 x 96 rxns)50 preps50 preps50 preps 250 preps250 preps32 columns 250 preps250 prepsTime 15 20 10 30 1030 10 5Elution volume 50 50150 520 30100 30100 50 20Recovery 80% 70% 80% Up to 95% Gel cleanup: 80%NA NAPCR cleanup: 95%Primer removal 99% NA 95% NA 99% NA 98%Thermo Scientific and Invitrogen technologies for plasmid DNA isolationPurity grade Molecular Transfection Transfection Advanced transfectionKits GeneJET kits PureLink HiPure kits PureLink Fast Low-Endotoxin kits PureLink Expi Endotoxin-Free kitsEndotoxin level Standard (10 EU/g) Low endotoxin (110 EU/g) Low endotoxin (0.11 EU/g) Endotoxin-free (0.1 EU/g)Yield 20 g1 mg 20 g15 mg 0.4 mg (midi), 1.5 mg (maxi) 1.515 mgTechnology Silica membrane Anion exchange (resin) Advanced silica membrane Anion exchange (membrane)Total protocol time 1560 min 30120 min 30 min 90120 minPrep size Mini, midi, and maxi Mini, midi, maxi, and giga Midi and maxi Maxi, mega, and gigaDownstream application PCR Standard transfection Standard transfection Primary and stem cell transfectionNucleic acid labeling All molecular biology applications Transfection of certain sensitiveGene therapy and vaccine cell lines (in vivo) researchCloningIn vitro transcription(digestion, ligation) MicroinjectionSequencing All molecular biology applicationsLearn more at thermofisher.com/plasmid 10 Contents'