b'Seamless cloning and GeneArt Gibson Assembly cloning kitsTo assemble multiple PCR fragments by end-terminal homologous recombination, several seamless cloning technologies are available for scarless and directional cloning into any vector. GeneArt seamless cloning kits offer the option of building constructs DNA fragments Linear vectorusing E. coli and Saccharomyces cerevisiae.Invitrogen GeneArt Gibson Assembly kits allow for the simultaneous assembly of up to 15 very large DNA fragments to create precise constructs with no additional sequences, in highly efficient reactions. This cloning method circumvents the need for multiple rounds of restriction enzyme analysis and digestion, DNA end repair, dephosphorylation, ligation, enzyme inactivation, and cleanup, and is a powerful tool in syntheticbiology.Assemble DNA fragmentsGeneArt Gibson Assembly kits offer these benefits: and linear vectorAssembly of up to 15 fragments to build seamless clonesCloning efficiencies up to 95% Choice of complete kits with competent cells or master mixesOneShot TOP10 chemically competentor ElectroMax DH10B electrocompetent E. coli transformation mixDid you know?The Gibson Assembly method has been referenced in thousands of peer- Cloningreviewed publications and is a powerful method that can be used to seamlessly Assembled construct synthetic and natural 1. Enzymatic assembly of DNA molecules up to several hundred kilobases. Gibson DG et al. (2009) Nat Methods 6(5):343-5. circular construct genes, genetic pathways, and entire genomes [1].Discover more at thermofisher.com/seamless Contents 43'