b'Cloning and gene synthesisFrom restriction enzymes to gene synthesis, a large portfolio of tools and resources is available to help you obtain high-quality cloned DNA for your next discovery.Invitrogen GeneArt Thermo Scientificseamless cloningInvitrogen GeneArt FastDigest restriction Invitrogen Gatewayand GeneArt GibsonInvitrogen GeneArtStrings DNAInvitrogen GeneArt Method enzymes TOPO cloning cloning Assembly cloning kits Type IIS assembly Fragments Gene SynthesisKey benefits/Familiarity, flexibility, 95% efficiency,High-throughput Seamless multi-One-tube seamless Synthesized DNA Custom-cloned genes description convenience, 5 min PCR cloningand high-efficiencyfragment assemblymulti-fragmentfragments ready toin your choice of time savings shuttling amongby homologousassembly byclone via the methodvectorCompatible with manymultiple expressionrecombinationsimultaneousof your choiceUniversal protocolother cloning systemsvectors restriction digestion Sequence-verifiedwith 15 min digestion Directional cloning ofand ligation No starting DNA in one buffer up to 15fragments required Can be optimizedDirectional cloning offor a specific hostUp to 95% efficiencyup to 8 fragments, for Pool sequence- for maximal protein and 15 min cloning up to 20 kb total verified expression Efficient for repetitive and very small sequencesTechnology Restriction digestion Topoisomerase-Single-step, End-terminal Type IIS restriction Linear dsDNA DNA of interest basics and ligation based, ligase-freedirectional, andhomologyand ligation in a assembled fromcloned in vectorcloning site-specific DNArecombinationsingle reaction pooled synthetic recombinationusing overlappingoligonucleotides100% sequence-sequences verified withRestriction enzyme- 1503,000 bp,quality assurance and ligase-freeTransformation- also available indocumentationassociatedlibrary format with recombination (TAR)randomized basesin Saccharomyces cerevisiaeNeeds DNA source material (gene inplasmid, library, etc.)Use your ownCloningvector** Vector needs to be converted with Invitrogen Gateway Vector Conversion System with One Shot ccdB Survival 2 T1R Competent Cells.Discover more at thermofisher.com/cloning Contents 39'