b'Restriction enzyme cloning Found naturally in bacteria, restriction enzymes recognize and cleave specific DNA sequences, resulting in sticky ends (5 or 3 protruding ends) or blunt ends, enabling DNA inserts to be cloned into vectors with compatible ends. Star activity, buffer compatibility, and varying protocols for complete digestion are some common hurdles in restriction digestion.FastDigest restriction enzymesTo simplify cloning, we offer FastDigest enzymesan advanced line of restrictionA Benzymes that share buffer compatibility with downstream modifying enzymes. ItsPlasmid DNA digested with FastDigest enzymes Plasmid DNA digested with NEB enzymesbenefits include: 1 2 3 4 5 6 7 8 9 1 2 3 4 5 6 7 8 9Complete digestion in 515 minDouble and multiple digestions in a universal buffer for any combination of enzymesNo sequential digestions and buffer changes 176 unique specificitiesDirect loading of reaction mixture on gels1: Undigested plasmid6: FastDigest XmaJI1: Undigested plasmid6: AvrII Find out more at thermofisher.com/fastdigest DNADNA 2: SpeI-HF7: FastDigest Eco31I7: BsaI 2: FastDigest BcuI3: XbaI 8: FastDigest Eco52I8: EaqI-HF 3: FastDigest XbaI4: NdeI 9: FastDigest BglII 9: BglI4: FastDigest NdeI5: SalI-HF 5: FastDigest SalI Comparison of digestion efficiencies of restriction enzymes. (A) FastDigest restriction enzymes digest plasmid DNA much more efficiently compared to the (B) NEB enzymes. In this experiment, using the NEB protocol, 1 g of plasmid DNA was digested in ~15 min. Incomplete digestion is shown in red boxes.Cloning40 Contents'