b'PCR primers and DNA oligos Good design (i.e., good sequence selection) andCustom DNA oligoshigh-quality primers are critical to your PCR reactions.Invitrogen custom DNA oligos are synthesized on a In general, a length of 1830 nucleotides for primers ishighly automated, computer-controlled system, followed optimal. The melting temperatures (T ) of the primersby rigorous quality control. Mass spectrometry and mshould be between 65C and 75C, and within 5C ofcapillary electrophoresis are performed for short andHelpful tipeach other. long oligos, respectively, to help ensure the quality of theIf the T mof your primer is very low, try to find a sequence process and end products.with higher GC content; alternatively, the length of the OligoPerfect primer designer primer can be extended. Whether your are performing PCR, cloning, or capillaryThe appropriate synthesis scale and purificationFor more tips on primer design, go to electrophoresis (CE) sequencing, take advantage offor your application depends on the nature of yourthermofisher.com/primerdesignthe benefits offered by our robust and easy-to-usedownstream applications. Choose the right oligos Primer3-based Invitrogen OligoPerfect Designer.and purification methods for your applications at thermofisher.com/oligos.PCRDid you know? Thermo Fisher Scientific offers scales beyond 10 mol up to kilograms, and employs a team of Speed updesign primers for up to manufacturing scientists dedicated to custom method 50 genes at the same time development for unique modifications. For information on large-scale and complex project capabilities, visit Store your dataability to save thermofisher.com/largescaleoligos.your projectsOption Desalted HPLC PAGEWork smarterrecognizes .txt andOligos 25 nmol10 mol 50 nmol10 mol 50 nmol10 mol.fasta file types 5100 bp 755 bp 7100 bpOrder with easeseamlessly integrates with85% full-length90% full-length sequence sequencethe Invitrogen ordering portal Standard PCR Specialty PCRTry the OligoPerfect Designer atCloningthermofisher.com/oligoperfect-designerIn addition to standard delivery, next-day delivery is also available in most regions where local oligo manufacturing exists. For more on ordering information, yield guarantees, designing tools, technical resources, protocols, and FAQs, go to thermofisher.com/oligos.Find out more at thermofisher.com/oligos 30 Contents'