b'Frequently asked questions (cont.) What do I need to run fast PCR? Nucleic acid electrophoresis CloningPCR amplicons shorter than 1 kb can be amplifiedWhy is it important to choose the right ladderDo you have a buffer compatibility chart for in as little as 40 minutes using fast enzymes (highwhen using E-Gel precast agarose gels? restriction enzymes?processivity; see page 28), fast plastics (low profile andAccurate analysis of electrophoresis bands oftenAll FastDigest restriction enzymes are 100%active in ultra-thin walls; see page 25), and fast thermal cyclersdepends on the DNA ladder chosen for your gel run.one universal FastDigest buffer (see page 40). Hence, (fast ramp rate; see pages 2223). E-Gel DNA ladders are formulated with ready-to-usethere is no buffer compatibility chart for FastDigest How can I prevent sample evaporationbuffers unique for E-Gel precast agarose gels, and DNArestriction enzymes.during PCR? standards designed for optimal separation (see page 34). What is the main difference between GeneArt Proper sealing of your reactions will help preventAre there safer alternatives to ethidium bromideStrings DNA Fragments and GeneArt Gene evaporation during PCR. for staining nucleic acids in gel electrophoresis? Synthesis?SYBR Safe DNA gel stain is a safer alternative to ethidiumGeneArt Strings DNA Fragments are custom-made, When using adhesive film to seal a plate, be surebromide and is commonly used in gel electrophoresis.uncloned, double-stranded linear DNA fragments. to properly align the seal to cover all wells and press firmly along all edges of the plate using anSYBR Safe DNA gel stain is not classified as hazardousGeneArt Gene Synthesis is a service offered for chemical applicator tool. waste or as a pollutant under US federal regulationssynthesis, cloning, and sequence verification of genetic When sealing a plate using cap strips, ensure that the(see page 36). sequences (see page 44).cap strips are compatible with the plate and thermalFor more tips and troubleshooting adviceWhat are some key considerations for choosing cycler being used. Be sure to align cap strips withon nucleic acid electrophoresis, visitcompetent cells for my cloning applications?each well of the plate and place firmly across the platethermofisher.com/na-electrophoresis-education andGenotype, transformation efficiency, growth rate, and for a secure fit.thermofisher.com/na-electrophoresis-support. throughput format are important factors in choosing Use the applicator tool (Cat. No. 4333183 or 4330015)competent cells for cloning. The genotype of a cell strain or other comparable sealing tools as needed.may determine growth conditions and suitability for For more tips and troubleshooting advice ontransformation with specific DNA types (see page 45).PCR, visit thermofisher.com/pcreducationFor more tips and troubleshooting advice on cloning, and thermofisher.com/pcrsupport. visit thermofisher.com/cloningeducation and thermofisher.com/cloningsupport.Resources52 Contents'