b'Reverse transcription primers The priming strategy you choose for reverse transcriptionFor target mRNA containing strong transcriptional is important for cDNA synthesis efficiency, consistency,pauses, random primers are better suited because they and yield. Each primer type has its benefits andanneal throughout the target molecules. They are also drawbacks, depending on the individual target RNA.ideal for nonpolyadenylated RNA, such as bacterial RNA.Reverse transcriptionFor full-length first-strand cDNA synthesis, oligo(dT) primers are recommended because of their specificity for eukaryotic mRNA, and they allow many different targets to be studied from the same cDNA pool. Oligo(dT) primers typically are strings of 1220 deoxythymidines. We offer oligo(dT) in different lengths and formats for flexibility in your reverse transcription experiments.Two most common primers used in reverse transcriptionHelpful tipTo avoid poly(A) slippage during priming, anchored oligo(dT) primers can be used to anneal to the 5 end of the poly(A) tail of mRNA and prevent priming within the poly(A) tail. Learn more about selection of primers for reverse transcription at thermofisher.com/rteducation.Learn more at thermofisher.com/rtprimers 18 Contents'