b'Genomic DNA removal RNA purification methods, including protocols withBoth the SuperScript IV One-Step RT-PCR System and DNase digestion on-column, often fail to removeSuperScript IV VILO Master Mix are available in a format gDNA completely. Amplification of contaminatingwith the novel dsDNA-specific Invitrogen ezDNase gDNA can cause inaccurate results. Traditional gDNAenzyme, which enables efficient, fast, and gentle (5 min decontamination protocols with DNase I includeat 37C) gDNA removal from RNA samples to help ensure transcription Reverse time-consuming DNase inactivation or removalhigh confidence in RT-PCR and RT-qPCR results. steps under conditions that can damage RNA and affect results.SuperScript IV and SuperScript IV VILO Master Mix cDNA synthesis workflow with ezDNase enzyme ~ 27RNAPrimerEnzyme27~~ 27minutesannealinginactivation minutminutes es(25C, 10 min)(85C, 5 min) RNRANAPriPmreimr erEnzEynmzyeme ezDNasaenn Reverse inaicntaivcatitvioantionaenanleinagli ng treatme(n2t5(2C5, 1C0, m10in m) in)transcrip(t8io5n(8C 5, 5C ,m 5in m) in) (37C, 2 min)(50C, 10 min) ezDeNzDasNea seRevReervseer se tretartemaetmntent tratnrsacnrsipctriopnti on (37(3C7, 2C ,m 2in m) in)(50(5C0, 1C0, m10in m) in) Traditional cDNA synthesis workflow with DNase I ~ 105~~ 0505RNADNase I DNase IPrimerReverseEnzyme11 minutestreatment inactivation/+EDTA annealingtranscriptioninactivationminut minutes es(37C, 20 min)(65C, 10 min)(25C, 10 min)(42C, 60 min)(85C, 5 min) RNRANADNDaNsea sI e I DNDaNsea sI e IPrimPreimr erRevReervseer seEnzEynmzyeme tretartemaetmntent inaicntaivcatitvioanti/o+nE/D+ETAD T Aannaenanleinagli ngtratnrsacnrsipctriopnti oninaicntaivcatitvioantion(37(3C7, 2C0, 2m0in m) in)(65(6C5, 1C0, m10in m) in)(25(2C5, 1C0, m10in m) in)(42(4C2, 6C0, 6m0in m) in)(85(8C5, 5C ,m 5in m) in) Learn more at thermofisher.com/ssiv-onestep and thermofisher.com/4vilo Contents 17'