b'Thermal cyclersThermal cyclers, which automate the heating and coolingwhich requires both accuracy and consistency in cycles required to amplify DNA, play a critical role in thethe thermal cycler block. If the temperature set success of PCR. The following are things to considerpoint of the instrument does not correspond to the when selecting a thermal cycler. actual temperature of the block, further temperature optimization could be required. Review a study ofHelpful tipPrecise temperature controltemperature accuracy in a number of models, available at Thermal cyclers with precise temperature controlthermofisher.com/thermalcycleraccuracy. Using the right PCR plastics for your application and instrument can improve enable you to quickly and accurately determine optimalthe reliability of your PCR results. Go to annealing temperatures. Several block technologies,Features thermofisher.com/findplastics to determine including gradient and Applied Biosystems VeriFlexA variety of Applied Biosystems thermal cyclers arethe right PCR plastics for you.Blocks temperature control, are available. A VeriFlexavailable to fit your applications and budget. Certain Block employs a separate heating and cooling elementfeatures may be important to you, depending on yourVeriFlex Block in each temperature zone, allowing better controlneeds. If you perform PCR optimization frequently, youtemperature zones PCRand precision of temperatures. Learn more about thewill likely benefit from an instrument with a VeriFlex Block. technology at thermofisher.com/veriflextechnology. If you would like to run optimized assays on a new or different thermal cycler, you can save re-optimization time Reliability by using a simulation mode.Thermal cyclers should be able to withstand repeatedIf you want remote access to your instrument, you will use, environmental stress, and shipping conditions.appreciate the convenience of cloud-enabled thermal Component reliability can be tested using roboticcyclers. They allow you to design and share protocols, assemblies in repeated testing of frequently usedschedule an instrument, start or stop a run, and check instrument components such as the heated lid,run status from anywhere, on any mobile device or touchscreens, and temperature cycling modules.desktop computer. Applied Biosystems thermal cyclers adhere to stringent reliability criteria, which are reported atFleet control thermofisher.com/thermalcyclerreliability.Applied BiosystemsIf you manage multiple thermal cyclers and users,Thermal Cycler Fleet you may benefit from a single interface for viewing allControl SoftwareTemperature accuracyThermal cycler temperature accuracy is a key factor ininstruments at a glance and setting custom permissions the success or failure of a PCR reaction. It is particularlyby instrument, user, and method. Learn more at important during annealing temperature optimization,thermofisher.com/fleetcontrol.Learn more at thermofisher.com/thermalcyclers Contents 21'