b'Reverse transcription reagent selection guide Ability to optimize reactionComplete kit withMost convenience and fewestMost convenience and fewestGo from mammalian cellcDNA synthesis and Reverse transcriptioncomponents and conditions all cDNA synthesispipetting steps for RT-qPCRpipetting steps for RT-PCRlysate to cDNA synthesisamplification directly from reaction components applications applications without having to isolate RNA intact single cells or low amounts of total RNAFirst-strand cDNA First-strand cDNA synthesis master mix for Product format Stand-alone enzyme synthesis kit RT-qPCR One-step RT-PCR kit Direct RT kit cDNA PreAmp kitInvitrogen SuperScript IV InvitrogenInvitrogen SuperScript IV RecommendedInvitrogenSuperScriptIVInvitrogenSuperScriptIV InvitrogenSuperScriptIVproductReverse Transcriptase First-Strand SynthesisVILO Master Mix One-Step RT-PCR System SuperScriptIV CellsDirect Single Cell/Low Input cDNA System cDNA Synthesis Kit PreAmp KitRT-PCR, RT-qPCR, cloning,RT-PCR, RT-qPCR, cloning, Applications cDNA library construction,cDNA library construction,RT-qPCR RT-PCR RT-PCR, RT-qPCR RT-qPCR, RNA-SeqRACE, RNA-Seq RACE, RNA-SeqInput total 1 pg5 g 1 pg5 g 0.01 pg2.5 g 0.01 pg1 g 110,000 cells 11,000 cells or 2 pg10 ngOptimal reaction5055C 5055C 5055C5055C 5055C 50CtemperatureReverse10 min 10 min 10 min 10 min 10 min 30 mintranscription timeHigh cDNA yield with challenging or degraded RNADid you know?The standard enzyme format is incompatible for lyophilization because of the glycerol in the storage buffer. The lyo-ready (lyophilization-ready) format of SuperScript reverse transcriptases has a glycerol content below 0.1% and offers greater stability for lyophilized molecular assay kits.Learn more at thermofisher.com/lyoreadyenzymes.Learn more at thermofisher.com/superscript 16 Contents'