b'Sample preparationSelecting the right DNA purification kits (cont.)Comparison of DNA cleanup solutionsDNA cleanupPCR cleanup Sequencingapplication PCR cleanup PCR cleanup PCR cleanup Gel extraction and gel extraction PCR cleanup reaction cleanupPureLink Quick Gel PureLink PCRPureLink Pro 96PureLink PCRPureLink QuickExtraction Kit and PCRChargeSwitch-ProCentri-Sep Spin Kit Purification Kit PCR Purification Kit Micro Kit Gel Extraction Kit Purification Combo Kit PCR Clean-Up Kit ColumnsFormat Silica spin/ 96-well silica plate Silica spinSilica spin/ Silica spin/ Derivatized spin/ Spin columnvacuum column column vacuum column vacuum column vacuum column 50 preps4 plates10 preps50 preps10 preps100 columnsProduct size250 preps (4 x 96 rxns)50 preps250 preps50 preps50 preps32 columns 250 preps250 prepsTime 15 20 10 30 1030 10 5Elution50 50150 520 30100 30100 50 20volumeRecovery 80% 70% 80% Up to 95% Gel cleanup: 80%NA NAPCR cleanup: 95%Primer99% NA 95% NA 99% NA 98%removalThermo Scientific and Invitrogen technologies for plasmid DNA isolationPurity grade Molecular Transfection Transfection Advanced transfectionKits GeneJET kits PureLink HiPure kits PureLink Fast Low-Endotoxin kits PureLink Expi Endotoxin-Free kitsEndotoxin level Standard (10 EU/g) Low endotoxin (110 EU/g) Low endotoxin (0.11 EU/g) Endotoxin-free (0.1 EU/g)Yield 20 g1 mg 20 g15 mg 0.4 mg (midi), 1.5 mg (maxi) 1.515 mgTechnology Silica membrane Anion exchange (resin) Advanced silica membrane Anion exchange (membrane)Total protocol time 1560 min 30120 min 30 min 90120 minPrep size Mini, midi, and maxi Mini, midi, maxi, and giga Midi and maxi Maxi, mega, and gigaDownstream applicationPCRStandard transfectionStandard transfectionPrimary and stem cell transfection Nucleic acid labelingAll molecular biology Transfection of certain sensitive Gene therapy and vaccineCloningapplications cell lines (in vivo) research(digestion, ligation)In vitro transcriptionMicroinjection SequencingAll molecular biology applications10'