b'Thermal cyclersThermal cyclers, which automate the heating andtemperature optimization, which requires both cooling cycles required to amplify DNA, play a criticalaccuracy and consistency in the thermal cycler role in the success of PCR. The following are things toblock. If the temperature set point of the instrument consider when selecting a thermal cycler. does not correspond to the actual temperature of the block, further temperature optimizationHelpful tipPrecise temperature controlcould be required. Review a study of temperatureUsing the right PCR plastics for your Thermal cyclers with precise temperature controlaccuracy in a number of models, available atapplication and instrument can improve enable you to quickly and accurately determine optimalthermofisher.com/thermalcycleraccuracythe reliability of your PCR results. Go to annealing temperatures. Several block technologies,thermofisher.com/findplastics to determine including gradient and Applied Biosystems VeriFlexFeatures the right PCR plastics for you.temperature control, are available. A VeriFlex BlockA variety of Applied Biosystems thermal cyclers are employs a separate heating and cooling element inavailable to fit your applications and budget. Certain each temperature zone, allowing better control andfeatures may be important to you, depending on yourPCRprecision of temperatures. Learn more about theneeds. If you perform PCR optimization frequently, you technology at thermofisher.com/veriflextechnology will likely benefit from an instrument with a VeriFlex Block. If you would like to run optimized assays Reliability on a new or different thermal cycler, you can save Thermal cyclers should be able to withstand repeatedre-optimization time by using a simulation mode.use, environmental stress, and shipping conditions. Component reliability can be tested using roboticIf you want remote access to your instrument, you will assemblies in repeated testing of frequently usedappreciate the convenience of cloud-enabled thermal instrument components such as the heated lid,cyclers. They allow you to design and share protocols, touchscreens, and temperature cycling modules.schedule an instrument, start or stop a run, and check Applied Biosystems thermal cyclers adhere torun status from anywhere, on any mobile device or stringent reliability criteria, which are reported atdesktop computer. thermofisher.com/thermalcyclerreliability If you manage multiple thermal cyclers and users, Temperature accuracy you may benefit from a single interface for viewing Thermal cycler temperature accuracy is a keyall instruments at a glance and setting custom factor in the success or failure of a PCR reaction.permissions by instrument, user, and method. Learn It is particularly important during annealingmore at thermofisher.com/fleetcontrolFind out more at thermofisher.com/thermalcyclers21'