b'Cloning and gene synthesisFrom restriction enzymes to gene synthesis, a large portfolio of tools and resources is available to help you obtain high-quality cloned DNA for your next discovery.Invitrogen GeneArt Thermo Scientificseamless cloningInvitrogenInvitrogenInvitrogen FastDigestInvitrogen Invitrogenand GeneArt GibsonGeneArt Type IIsGeneArt StringsGeneArt Gene Method restriction enzymes TOPO cloning Gateway cloning Assembly cloning kits assembly DNA Fragments SynthesisKey benefits/Familiarity, flexibility, 95% efficiency,High-throughput Seamless multi-One-tube seamless Synthesized DNA Custom-cloned description convenience, 5 min PCR cloningand high-efficiencyfragment assemblymulti-fragmentfragments readygenes in your time savingsCompatible withshuttling amongby homologousassembly byto clone via thechoice of vectorUniversal protocolmany other cloningmultiple expressionrecombinationsimultaneousmethod of your Sequence-verifiedwith 15 min digestionsystemsvectorsDirectional cloning ofrestriction digestionchoice in one buffer up to 15fragments and ligation No starting DNA Can be optimizedDirectional cloningrequiredfor a specificUp to 95% efficiencyof up to 8 fragments,host for maximal and 15 min cloning for up to 20 kb totalPool sequence- protein expressionverified Efficient for repetitive and very small sequencesTechnology basicsRestriction digestion Topoisomerase-Single-step, End-terminal homology Type IIs restriction Linear dsDNA DNA of interest and ligation based, ligase-freedirectional, andrecombination usingand ligation in a assembled fromcloned in vectorcloning site-specific DNAoverlapping sequences single reaction pooled synthetic 100% sequence-recombination Transformation- oligonucleotides verified withRestrictionassociated 1503,000 bp,quality assurance enzyme andrecombination (TAR)also available indocumentationligase-free in Saccharomyceslibrary format with cerevisiae randomized basesNeeds DNA source material (gene inplasmid, library, etc.)Use your own vector** Vector needs to be converted with Invitrogen Gateway Vector Conversion System with One Shot ccdB Survival 2 T1R Competent Cells.CloningFind out more at thermofisher.com/cloning 39'