b'Choose the right PCR reagent for your research needs A comprehensive portfolio of PCR enzymes and master mixes is available with the high performance and consistency you need. Start with the selection guide below to find the best enzyme for common PCR applications.Invitrogen Platinum Invitrogen Platinum Invitrogen Platinum II TaqApplied Biosystems AmpliTaqDirect PCR Universal DNA polymerase SuperFi II DNA Polymerase Hot-Start DNA Polymerase Gold 360 DNA Polymerase Master MixPCR type High-fidelity PCR Hot-start PCR Hot-start PCR Direct PCRCapabilities Highly accurate ampliconUniversal primer annealing, fastChemical hot start Detection of target DNA sequences, universal primerDNA synthesis, detection of low- without genomic DNA annealing, robust amplificationabundance targets purificationof difficult targetsTechnical specificationsFidelity compared to Taq polymerase 300x 1x 1x 1xPCRTarget length Up to 20 kb* Up to 5 kb Up to 5 kb Up to 8 kbHot-start modification Antibody-mediated Antibody-mediated Chemical modification Antibody-mediatedSpeed 1530 sec/kb 15 sec/kb 60 sec/kb 20 sec/kbUniversal primer annealing Yes Yes YesInhibitor tolerance Yes Yes YesBlunt or 3-A end Blunt 3-A 3-A 3-ACompatible with Applied Biosystems TaqMan probes Yes YesCertified low level of bacterial gDNAYes Yes YesApplicationsCloning and subcloning Site-directed mutagenesis GC-rich amplificationTemplate generation for sequencingHigh-throughput PCR Long PCR (up to 20 kb) GenotypingAmplification of samples with suboptimal purity Colony PCRMultiplex PCRFast PCR * Amplification of up to 40 kb fragment sizes is possible, but may require additional optimization of reaction conditions and primer design.28'