b'Reverse transcription primers The priming strategy you choose for reversethe same cDNA pool. Oligo(dT) primers typically transcription is important for cDNA synthesisare strings of 1220 deoxythymidines. We offer efficiency, consistency, and yield. Each primer typeoligo(dT) in different lengths and formats for has its benefits and drawbacks, depending on theflexibility in your reverse transcription experiments.Reverse transcriptionindividual target RNA. For target mRNA containing strong For full-length first-strand cDNA synthesis,transcriptional pauses, random primers are oligo(dT) primers are recommended because ofbetter suited because they anneal throughout their specificity for eukaryotic mRNA, and theythe target molecules. They are also ideal for allow many different targets to be studied fromnonpolyadenylated RNA, such as bacterial RNA.Two most common primers used in reverse transcriptionHelpful tipTo avoid poly(A) slippage during priming, anchored oligo(dT) primers can be used to anneal to the 5 end of the poly(A) tail of mRNA and prevent priming within the poly(A) tail. Learn more about selection of primers for reverse transcription at thermofisher.com/rteducationFind out more at thermofisher.com/rtprimers 18'