b'Antibody-based hot-start DNA polymerase and its activation in PCR for enhanced specificityHelpful tipOne of the most commonPCR troubleshooting issues is the presence of unwanted bands, or nonspecific amplification. To reduce nonspecific amplification:Optimize annealing temperatureCheck primer design PCRUse hot-start PCRPrevent DNA cross-contaminationDecrease template and/or primer concentrationOptimize Mg 2+concentrationInactive Activated27'