b'Seamless cloning and GeneArt Gibson Assembly cloning kitsTo assemble multiple PCR fragments by end-terminal homologousLinear vectorrecombination, several seamless cloning technologies are availableDNA fragmentsfor scarless and directional cloning into any vector. GeneArt seamless cloning kits offer the option of building constructs using E. coli and Saccharomyces cerevisiae.Invitrogen GeneArt Gibson Assembly kits allow for the simultaneous assembly of up to 15 very large DNA fragments to create precise constructs with no additional sequences, in highly efficient reactions. This cloningAssemble DNA fragmentsand linear vectormethod circumvents the need for multiple rounds of restriction enzyme analysis and digestion, DNA end repair, dephosphorylation, ligation, enzyme inactivation, and cleanup, and is a powerful tool in syntheticbiology.GeneArt Gibson Assembly kits offer these benefits:OneShot TOP10 chemically competent Assembly of up to 15 fragments to build seamless clones or ElectroMax DH10B electrocompetent E. coli transformation mix Cloning efficiencies up to 95%Did you know? Choice of complete kits with competent cells or master mixes The Gibson Assembly method has been referenced in thousands of peer- Cloningreviewed publications and is a powerful method that can be used to seamlessly Assembled construct synthetic and natural genes, circular construct genetic pathways, and entire genomes. 1Find out more at thermofisher.com/seamless 1. Enzymatic assembly of DNA molecules up to several hundred kilobases. Gibson DG et al. (2009) Nat Methods 6(5):343-5. 43'