b'Molecular cloning involves recombinant DNA technologies that insert a DNA sequence of interest into a vector to generate a large number of copies. Traditionally, cloning has been carried out with restriction enzymes and a DNA ligase to form a new vector capable of expressing the gene of interest. In the case of gene synthesis, researchers can obtain their desired DNA directly in a specified vector with just sequence information. Other cloning methods, such as PCR cloning, InvitrogenTOPOcloning,ligation-independent cloning, and gene assembly are commonplace, exploiting unique characteristics of other DNA-modifying enzymes.Find technical resources on molecular cloning atthermofisher.com/cloningeducation38'