b'TransformationOnce the DNA fragment is cloned into a vector, transformation into bacteria is performed to enable propagation of sufficient quantities of the cloned DNA for downstream experiments. Selection of competent cells for transformation depends upon the transformation methods, strain genotypes, plasmid characteristics, and desired applications. Visit thermofisher.com/compcells-education for technical resources on competent cells.Choosing Invitrogen competent cells based on the applicationIs this for routine cloning and subcloning?Yes NoRoutine cloning SpecializedProtein cloningproductionYes NoIs high transformationIs this for gDNA or efciency needed? library production?Yes No Yes No Contact technical Protein production supportthermosher.com/High transformationStandard efciencyOne Shot BL21 Star(DE3)contactefciency (cloning) (propagation) Is this forHigh mRNA stability forgDNA or library unstable inserts? increased protein expressionOne Shot OmniMAX 2 T1RSubcloning Efciency(5 x 109 cfu/g)DH5 (1 x 106 cfu/g) production Yes No One ShotBL21(DE3) High-level expressionOne Shot TOP10One Shot OmniMAX 2 T1Rof recombinant proteins(1 x 109 cfu/g)(20 kb)Unstable inserts Is this for methylation- One Shot BL21(DE3) pLysE One Shot DH10B T1RElectroMAX DH10B sensitive plasmid DNA? Lower basal expression(1 x 109 cfu/g) (20 kb)MAX Efciency Stbl2Yes No of T7 promoter genesMAX Efciency DH5 ElectroMAX DH10B T1ROne Shot Stbl3 One Shot BL21(DE3) pLysS (1 x 109 cfu/g) (20 kb)(lentiviral vectors)Low basal expression, faster RIs this for Invitrogeninduction of T7 promoter genesLibrary EfciencyDH5 * MegaX DH10BT1 ElectroMAXStbl4 (1 x 108 cfu/g) Electrocomp (100 kb)Gatewaydestination One Shot BL21-AIvector propagation?One Shot Mach1-T1RBest for expression of Lorem ipsum(1 x 109 cfu/g) Methylation-sensitive Yes No toxic proteinsCloningOne Shot INV110Elec trocompet tent ce ells.Gateway destinationContact technical Elec trocompe ent c lls.* Not fo r me hylated or gen nomDNA A. vectorsupport* Not fo r met thylated or ge omic ic DN . thermosher.com/One Shot ccdBcontactSurvival 2 T1RFind out more at thermofisher.com/compcells 45'