b'Reverse transcription reagent selection guide Ability to optimizeComplete kit withMost convenience andMost convenienceGo from mammalian reaction componentsall cDNA synthesisfewest pipetting steps forand fewest pipettingcell lysate to cDNA Reverse transcriptionand conditions reaction components RT-qPCR applications steps for RT-PCRsynthesis without having applications to isolate RNAFirst-strand cDNA First-strand cDNA synthesis Product format Stand-alone enzyme synthesis kit master mix for RT-qPCR One-step RT-PCR kit Direct RT kitRecommendedInvitrogen SuperScript IVInvitrogen SuperScript IV Invitrogen SuperScript IV Invitrogen SuperScript IV Invitrogen SuperScript IV productReverse Transcriptase First-Strand Synthesis System VILO Master Mix One-Step RT-PCR System CellsDirect cDNA Synthesis KitRT-PCR, RT-qPCR, cloning,RT-PCR, RT-qPCR, cloning,Applications cDNA library construction,cDNA library construction,RT-qPCR RT-PCR RT-PCR, RT-qPCRRACE, RNA-Seq RACE, RNA-SeqInput total 1 pg5 g 1 pg5 g 0.01 pg2.5 g 0.01 pg1 g 110,000 cellsOptimal reaction5055C 5055C 5055C5055C 5055CtemperatureReverse10 min 10 min 10 min 10 min 10 mintranscription timeHigh cDNA yield with challenging or degraded RNADid you know?The standard enzyme format is incompatible for lyophilization because of the glycerol in the storage buffer. The lyo-ready (lyophilization-ready) format of SuperScript reverse transcriptases has a glycerol content below 0.1% and offers greater stability for lyophilized molecular assay kits.Learn more at thermofisher.com/lyoreadyenzymesFind out more at thermofisher.com/superscript 16'