b'SeparateTransferDetectGet high-efficiency transfers in a fraction of the timeA successful western blot experiment relies on the quality of transfer of proteins from your gel to the blotting membrane. Quality of transfer is dependent on transfer efficiency and consistency. Traditional wet transfer off ers high efficiency, but at a cost of time and hands-on eff ort. The iBlot2 system off ers the same high quality of transfer but with signifi cant improvements in speed and convenience. Traditional wet tank60 min transferEGFRHsp90TubulinTraditional wet tank iBlot 2 system60 min transfer 7 min transfer p23A431 lysateEGFR iBlot 2 system7 min transferHsp90PDI EGFRHsp90Tubulinp23p23Chemiluminescent detection: high transfer ecienciesMultiplex fl uorescent detection: high transfer eciencies achieved using the iBlot 2 Dry Blotting System. A431achieved using the iBlot 2 Dry Blotting System. A431 lysate lysate was separated on InvitrogenNovex Tris-glycine gelswas separated on NuPAGE Bis-Tris gels and transferred to and transferred to nitrocellulose using a traditional wet tanknitrocellulose using a traditional wet tank system or the iBlot2 system or the iBlot 2 Gel Transfer Device. The membraneGel Transfer Device. The membrane was probed for EGFR, was probed for EGFR, Hsp90, PDI, and p23 and detectedHsp90, tubulin, and p23.using Thermo Scientifi c SuperSignal West Dura Extended DurationSubstrate. 11'