b'SeparateTransferDetectHigh-efficiency western blottingiWestern workfl owAt the core of the iWestern workfl ow bundle are four innovative products optimized to deliver the performance you expect from us. These products work together to help you achieve exceptional western blotting results with minimal hands-on time. Western blot analysis in as little as 4 hours20 minutes 7 minutes 3 hours FinishedInvInitvroitgroegnen Booltl InInvivtritoroggenen iBlot 22D Grye Tl rTarannsfsefer rD Deveivciec eInInvvitirtoroggeenn iBind Wesetsetrenr nS Sysytsetmemss InIvnivtritorogegneImagaginigng S Sysytsetemmss B tBBiissTTrriissPPlluussGGeellssW n iBright Imwith Invitrogen Mini Gel TankWestern steps iWesternManual Sample prep/ 50 min 90 mingel electrophoresisTransfer 7 min 75 minBlocking 60 minPrimary incubation 180 min 720 minWashes 60 minSecondary incubation 60 minSubstrate incubation 5 min 5 minTarget detection 2 min 5 minTotal time 244 min (approx. 4 hr) 1,075 min (approx. 18 hr)Compare workfl ow and data obtained using the iWestern workfl ow bundle and manual western blotting. The iWestern workfl ow enables the detection of HDAC1 in HeLa lysate in approximately 4 hr vs. 18 hr using the manual western workfl ow. Two-fold serial dilutions (starting with 20 g of HeLa lysate) were loaded and separated on Tris-glycine gels. The proteins were transferred to nitrocellulose membranes and detected using Thermo Scientifi c SuperSignal West Pico PLUS chemiluminescent substrate.Customize your bundle at thermofi sher.com/iwestern6'