b'SeparateTransferDetectFrustrated by smiling or uneven bands? The most widely used gel system for separating aUnlike traditional Tris-glycine gels, NuPAGE and Bolt gels broad range of proteins by SDS-PAGE is the Laemmliare Bis-Tris HCIbuffered (pH 6.4) and have an operating system (Tris-glycine). The highly alkaline operating pHpH of about 7.0. The neutral operating pH of the Bis-Tris of the Laemmli system may cause band distortion,systems provides the following advantages over the loss of resolution, or artifact bands as a result ofLaemmli system:protein degradation. Longer shelf life of 816 months due to improved The major causes of poor band resolution with thegel stabilityLaemmli system are:Improved protein stability during electrophoresis atHydrolysis of polyacrylamide at the high pH of theneutral pH, enabling sharper band resolution and resolving gel, resulting in a short shelf life of 8 weeks accurate results Chemical alterations such as deamination and alkylation Complete reduction of disulfides under mild heating of proteins due to the high pH of the resolving gel conditions (70C for 10 minutes) and absence of cleavage of Asp-Pro bonds Reoxidation of reduced disulfides from cysteine-containing proteins, as the redox state of the gel is Reduced state of the proteins maintained during not constant electrophoresis and blotting of the proteins when using Invitrogen NuPAGE and Bolt Antioxidant Cleavage of Asp-Pro bonds of proteins when heated at 100C in Laemmli sample buffer, pH 5.2Purchase with confidence with our NuPAGE Bis-Tris chemistry with neutral pH minimizesprotein gel performance guaranteeprotein distortion during the electrophoresis run.We stand behind the quality of our A B high-performance protein gels. Our manufacturing 1234 5678 9 101234 5 67 8 9 10 and quality assurance teams are highly trained andour product specifications are unmatched. You can purchase with confidence, knowing that we back up the quality of our protein gels with the Invitrogen protein gels performance guarantee. If an Invitrogen protein gel does not perform in your experiment as NuPAGE Bis-Tris gel Bio-Rad TGX gel described on our website or Certificate of Analysis, we Protein separation using a NuPAGE Bis-Tris gel and a Bio-Rad TGXwill replace the product at no cost to you, or we will gel. Samples were run on (A) a NuPAGE 412% Bis-Tris protein gel inprovide you with a credit for future purchase. MES SDS running buffer or (B) a TGX 420% Tris-glycine gel. Note the high resolution of the sample bands in the NuPAGE protein gel. The poorly resolved fuzzy bands seen in the other suppliers gel are a result ofFind out more at reoxidation of some disulfide bonds within the sample, leading to slightthermofisher.com/proteingelguaranteechanges in migration rates.39'