b'SeparateTransferDetectLet your colors show with iBright Imaging Systems The iBright FL1500 model features fi ve fl uorescenceFilter sets are pre-installed in iBright FL1500 Imaging channels, permitting up to four-color fl uorescentSystems for visible light range (RGB) and NIR western blot multiplexingexpanding your possibilitiesfl uorescent western blotting applications.for studying multiple proteins in a single blot. Obtain Example meaningful and representative comparisons to helpcompatibleExcitationFilterEmissionFilter enhance your experiments. Built-in SmartExposurefl uorophores channel range (nm) channel range (nm)Alexa Fluor Plus 488, technology further improves acquisition of multiplexAlexa Fluor 488 EX1 455485 EM1 508557fl uorescent western blot data, because signal-to- Alexa Fluor Plus 555,EX2 515545 EM2 568617noise ratios are optimized for each fl uorescenceAlexa Fluor 546Alexa Fluor Plus 647,EX3 608632 EM3 675720channel separately. Alexa Fluor 594Alexa Fluor Plus 680,EX4 610660 EM4 710730Alexa Fluor 680Alexa Fluor Plus 800,EX5 745765 EM5 800850Alexa Fluor 790One-color fl uorescence blot Two-color fl uorescence blot Three-color fl uorescence blotFour-color fl uorescence blot Alexa Fluor Plus 488 dyeRB1Alexa Fluor 546 dyecalreticulin protein with GST and HA tagprotein (55kDa)(134kDa)Alexa Fluor Plus 680 dyep23Alexa Fluor Plus 800 dyeHSP90MembraneiBright prestainedmarkerprotein (23kDa) protein (90kDa)Four-color multiplexed fl uorescent blot: false color composite top left, and individual channels shown in grayscale as pictured.32'