b'SeparateTransferDetectChoose the right SuperSignal substrate for your needs.SuperSignalSuperSignalSuperSignalSuperSignal West Pico PLUS West Dura West Femto West AttoDetection level Low-picogram toMid-femtogram Mid- to low-femtogram Low-femtogram to high-attogramhigh-femtogram Signal durationUp to 24 hours Up to 24 hours Up to 8 hours Up to 6 hoursAntibody 1: 1:1K5K 1: 1:1K50K 1: 1:5K100K 1: 1:1K1:5Kdilution 2: 1:10K1:100K 2: 1:50K1:250K 2: 1:100K1:500K 2: 1:100K1:250KSuperior sensitivity andBest for use with imagingMost sensitive substrate for HRP Advantages intensity and longer durationequipment; long-lastingGood sensitivity withdetection with high signal-to-than other entry-level ECLsignal duration optimized conditions noise ratiosubstratesRoutine western blots;Target is very low in abundance/working with new proteinMaximum signal duration isTarget is low in abundance/ sample is precious; requires Select when: target when western blottingneeded; performing sensitivesample is precious; systemmaximum sensitivity with less conditions are not yetquantitation is optimized optimizationoptimizedThermo Scientifi c SuperSignal West Atto Ultra-sensitivitylow-femtogram to high-attogramlevelsUltimate Sensitivity Substrate provides the highest sensitivity and improved signal-to-noise High signal-to-noise ratioexhibits superior intensity ratio compared to other commercially availableand sensitivity versus other typical high-performance ECL high-performance horseradish peroxidase (HRP)substrates on the marketsubstrates. It is the ideal choice for detection of very Excellent signal durationup to 6 hours of usable light low-abundance targets or precious samples thatoutput when conditions are optimized require maximum levels of sensitivity. Stable reagentworking solution is stable for 48 hours; kit is stable at 28C for 12 months5 2.5 1.25 0.625 0.312 0.156Signal-to-noise ratio of ECL substratesgSuperSignal West Atto 2.2 SuperSignal West AttoBio-Rad Clarity Max2.0Bio-Rad Clarity Max Amersham ECL Select1.8 Immobilon WesternSignal-to-noise ratioAmersham ECL Select Bio-Rad Clarity1.6 GE Healthcare ECLImmobilon Western1.4Bio-Rad Clarity1.2GE Healthcare ECL1.00.625 g 0.313 g 0.156 gHeLa lysate protein43'