b'SeparateTransferDetectBlot with confidence with high-quality primary and secondary antibodies Find an extensive selection of high-quality Invitrogen antibodies for western blotting, available in a wide range of targets and hosts. Explore our cross-adsorbed secondary HRP antibodies that help decrease species cross-reactivity and minimize background. Purchase with confidence thanks to our antibody performance guarantee.* Find the right antibody for your research needs atHRP Secondary Antibodythermofisher.com/antibodies* Terms and conditions apply. For complete details, go to thermofisher.com/antibody-performance-guarantee.Avoid interference from IP antibodies A B C DClean-Blot Clean-BlotWhen performing immunoprecipitation (IP), target proteinA B C D Detection Detection signal can be hindered by the heavy and light chains fromGAR-HRP Reagent GAR-HRP ReagentIgGs (~50 kDa and 25 kDa). Western blot interference from IgGs results from IP methods that release the antibody with the antigen and use sample types that contain IgGs, suchNFBas tissue extracts. Traditional secondary antibodies will detect the denatured and blotted IgGs.BaxThermo Scientific Clean-Blot IP Detection Reagent Lysate Lysate Lysate Lysateis a horseradish peroxidase conjugate that is optimizedWash Elute Wash Elute Wash Elute Wash Elutefor post-IP western blot detection of primary antibodiesNFB and Bax were immunoprecipitated from A549 lysate using without interference from denatured IP antibodyProtein A/G agarose resin and rabbit anti-NFB (panels A and B) and rabbit anti-Bax (panels C and D). Panels A and C were detected with fragments. The Clean-Blot IP reagent minimizes detectiongoat anti-rabbit HRP, which masked the targets. Panels B and D were interference from both heavy-chain and light-chain IgGdetected with the Clean-Blot IP Detection Reagent (HRP), unambiguously fragments of antibodies by only detecting the nativerevealing the target protein without interference.antibodies, providing accurate and specific detection of the target antigen.44'