b'SeparateTransferDetectFlexible protein transferPower Blotter Transfer SystemPower Blotter XL Welcome Pack (Cat. No. PB0113)The Invitrogen Power Blotter System features interchangeable blotting cassettes to suit your required throughput, and multiple transfer-stack consumable choices. Designed for rapid 510 minPower Blotter Systemtransfer of proteins from polyacrylamide gels to nitrocellulose or PVDFTransfer 12 mini-size gels or membranes, the Power Blotter utilizes an integrated power supply, LCD1 midi-size geltouchscreen, and preprogrammed, optimized transfer protocols.Versatilecan be used with do-it-yourself, ready-to-build, or ready-to-use transfer stacks Ecienthigh transfer efficiency for a broad range of protein sizesPower Blotter XL System High throughputtransfer up to 4 mini or 2 midi gels simultaneouslyTransfer 14 mini-size gels or with the Power Blotter XL System 2 midi-size gelsDesigned to grow around your labs needs The Power Blotter platform off ers options based on throughput requirements. Both cassettes are interchangeable with the Invitrogen Power Blotter Station power supply and control base.The Power Blotter off ers high transfer eciency for a broad range of protein sizesApparatus and consumables Power Blotter with Select Transfer Stacks Power Blotter with Pre-cut Membranes and Filters and Power Blotter 1-Step Transfer Buff erTransfer time 510 min 510 minKLH KLHEGFR EGFRHsp90 Hsp90PDI PDIEGFR EGFRTransfer eciency (extra band) (extra band)Cyclophilin B Cyclophilin BInvitrogen Power Blotter Select Transfer Stacks and Power Blotter Pre-cut Membranes and Filters eciently transfer high, medium, and low molecular weight proteins. Western blot analysis of several targets (KLH, EGFR, Hsp90, PDI, and cyclophilin B protein) was performed by loading serially diluted HeLa cell lysate with KLH spike (starting at 7.5 g HeLa lysate, 7.5 g KLH spike per well, serially diluted 2:3) onto Bolt 412% Bis-Tris Plus gels. Proteins were transferred in 7 min using a Power Blotter Select Transfer Stack (left, Cat. No. PB5310 and PB3310) or Power Blotter Pre-cut Membranes and Filters (right, Cat. No. PB9320 and PB7320), probed with target-specifi c primary antibodies and fl uorescently conjugated secondary antibodies. Images were captured using automatic exposure on an iBright FL1000 Imaging System.Find out more at thermofi sher.com/powerblotter22'