b'SeparateTransferDetectPrestained fl uorescent laddersDuring western blotting, prestained and fl uorescent protein ladders can be used for approximate determination of molecular weight, monitoring the progress of electrophoresis runs, and/or estimating the efficiency of protein transfer to the membrane during western blotting. We off er several prestained and fl uorescent protein ladders supplied in a ready-to-use format to facilitate easy protein analysis during gel electrophoresis and fl uorescent western blotting.kDa kDa kDa kDa250 ~250 250~130 130130 ~100 95Visible light Near-IR imager Gel: 420% Tris-glycine (SDS-PAGE) Visible light Blot: Chemiluminescent detection Blot: Near-IR detection95 ~70 807070 ~55 5555 4343 ~3534 34 30~25 262615 ~15 1511 ~10 10Gel: 420% Tris-glycine 680 nm 550 nm Gel: 420% Tris-glycine(SDS-PAGE) (SDS-PAGE)Thermo Scienti c PageRulerThermo Scienti c PageRulerInvitrogen iBright Prestained Prestained NIR Protein Ladder Plus Prestained Protein Ladder Protein Ladder Ten blue-stained proteins; Nine color-stained proteins, Ten blue-stained proteins; also also fl uorophore-labeled toproviding NIR fl uorescencefl uorophore-labeled to enable enable direct and near-IRwith seven bands, and RGBdirect and NIR fl uorescent (NIR) fl uorescent visualizationfl uorescence (550 nm channel)visualizationwith the two orangebandsTwo unstained proteins (30 kDa and 80 kDa) that enable visualization with chemiluminescent or fl uorescent detectionLearn more at thermofi sher.com/proteinladders27'