b'SeparateTransferDetectOvercome high backgroundBlocking bu ers Before probing for proteins of interest, the remaining binding surface of the membrane must be blocked to prevent nonspecifi c binding of the antibodies. Otherwise, the antibodies or other detection reagents will bind to any remaining sites on the membrane that initially served to immobilize the proteins of interest. There is no single blocking buff er that can universally work for all western blotting applications. Refer to the table below to choose the optimal blocking buff er for your application. Thermo Scientifi cBlockingAvailable Select when . product agent HighlightsWhen to use formats With medium- to high- Performs well with a wide range ofabundance proteins oroptimizing a Serum andstrong antibody affinityPBS StartingBlock biotin-freeantibodies and antibody combinations TBSnew western blotBlocking Buff er single purifi ed Compatible with streptavidin systemsCurrent blocking buff erPBSTsystem proteinhas high background TBST Blocks in less than 15 minStripping and reprobing western blots Blocks excess nonspecifi c binding sites to help reduceperformingBlocker FLbackground fl uorescencefl uorescentFluorescent BlockingSingle purifi ed Works with both nitrocellulose and Imaging and storage of dry10X westernBuff er (10X) protein low-fl uorescence PVDF membranes fl uorescence blots concentratedetection Detergent-free Blocks in 1530 min you have high Clarifi ed and High-performance replacement for background withPierceClear Milkstabilized milkhomemade milk blocking buff ers in Use when high background10X homemade milkBlocking Buff er proteinswestern blotting applicationsis seen with nonfat milk concentratebuff ersLong shelf life at room temperature 10% solutions of high-quality bovine serum albuminUse when targetingtargetingBlocker BSA Purifi ed bovine Single purifi ed protein providesphosphoproteins 10X phosphoproteins serum albumin fewer chances of cross-reaction with Best to use when storingconcentrateassay components than serum orreused antibodies in blockermilk solutions Helps minimize or eliminate cross-reactivity associated with protein-based blocking buff ersPBS. you need aPierce Protein-FreeNon-protein Sample-and-antibody combinations Use when protein-basedTBSprotein-freeBlocking Buff er blockingrequire the elimination of all possibleblockers cause highPBSTblocker compound exogenous animal proteins inbackground TBSTthe assay system to avoid cross-reaction or quenching of the desired probefunctionFind out more at thermofi sher.com/westernbuff ers41'