b'SeparateTransferDetectFluorescence-compatible samplebuffers Sample buffers containing bromophenol blue will fluoresce and can contribute to increased background. While the dye front may be run off the gel prior to transfer or cut from the membrane after transfer to avoid background fluorescence signal, it is ideal to use fluorescence-compatible sample buffers without bromophenol blue, such as Invitrogen Fluorescent Compatible Sample Buffer. Nonreducingready to use for nonreducing SDS-PAGE, or add the preferred type and amount of reducing agent (e.g., DTT) to produce reducing conditions Lane marker dyesvisible dye front to monitor run Convenientstable at room temperature, enabling storage at the bench where electrophoresis is performedFluorescence-compatible bu\x0eerSample bu\x0eers containing bromophenol blueHeLa lysates prepared with Fluorescent Compatible Sample Buffer or with Novex Tris-Glycine SDS Sample Buffer. Samples were separated on a Tris-glycine gel and transferred to a nitrocellulose membrane. Image was captured using the appropriate filter sets for Near-IR dyes at 680 nm.680 nmLearn more at thermofisher.com/5steps-multiplexwestern26'