b'SeparateTransferDetectFluorescent-labeled antibodiesGet clean, multiplexeduorescent western blots Avoid cross-reactivityuse our secondary with Invitrogen cross-adsorbed secondaryantibodies that are highly cross-adsorbed against antibodiesserum proteins or IgG of other species Cross-adsorbed secondary antibodies are polyclonal antibodies that are manufactured with an additional Highly purifi edour secondary antibodies are highly purifi cation step to fi lter out members that bindpurifi ed and manufactured for lot-to-lot consistency to off -target species of immunoglobulin (IgG). The Alexa Fluor and Alexa Fluor Plus secondary process decreases species cross-reactivity andantibodiesget distinct fl uorescent bands in increases specifi city.multiplex experiments due to easy selection of non-overlapping spectra from a variety of conjugatesWe off er a wide range of cross-adsorbed, specifi c antibody conjugates for target and host species, Easy visualizationclearly detect independent including Invitrogen Alexa Fluor, Alexa Fluor Plus, andbands in the same lane and blotclassical fl uorophores (FITC, TRITC, Cy3, Cy5, Cy5.5, Sensitive detectionuse Alexa Fluor Plus secondary APC, etc.). Alexa Fluor conjugates come in 19 colorsantibodies for detection of low-abundance proteinsand 45 host/target species combinations. Alexa Fluor Plus conjugates come in 6 colors and 6 host/target species combinations.EGFA431 control A431 Cas9A431 EGFR KOcontrol(200 ng/mL, 10 min) 1 2 1 2 1 2Overlay: 160 kDa p-EGFREGFR 160 kDa p-EGFR 160 kDa EGFR Overlay: 45 kDa p-MEK1 MEK137 kDa GAPDH45 kDa p-MEK145 kDa MEK1 Alexa Fluor 37 kDa GAPDH SecondaryAntibodyFluorescent western blot analysis of p-EGFR, EGFR, p-MEK1, MEK1, and GAPDH in A431 control, Cas9, and EGFR KO cells. Targets were detected using Alexa Fluor Plus donkey anti-rabbit 680 and 800 conjugates and Alexa Fluor Plus donkey anti-goat 488 and 555 conjugated secondary antibodies (Cat. No. A32814 and A32816).Search for your secondary antibodies at thermofi sher.com/secondaries30'