b'SeparateTransferDetectLight up weak or faint bandsGet better sensitivity with the rightDont know where to start? chemiluminescent substrateTry Thermo Scientifi c SuperSignal chemiluminescent As with other components in a western blotting system,substrate bundles.there are many chemiluminescent substrate choices available. The appropriate substrate selection depends on the detection level (sensitivity) required, the target protein abundance, and the sample availability.Thermo Scientifi c SuperSignal West Pico PLUS Chemiluminescent Substrate provides excellent performance, versatility, and economy for routine western blotting needs. It is designed to work for the majority of western blots and recommended for detecting a new protein of interest when western blotting conditions are not yet optimized.Excellent sensitivitylow-picogram to high-femtogramsensitivity Long signal durationup to 24 hoursExceptional robustnesshigh performance, even outside of the recommended antibody ranges, includingStable reagent8 hour working solution stability,the most commonly used 1:5,000 to 1:10,000 secondary 1 year kit stability at room temperature antibody dilutions from a 1 mg/mL stock solutionSuperSignal WestPico PLUSBio-Rad ClarityTarget: beta-CateninelF4ERSK2Ezrin MW: 65 kDa 25 kDa 80 kDa 69 kDaLysate (amount): HEK293 (4 g) HEK293 (40 g) HeLa (20 g) HeLa (20 g)Membrane: PVDF PVDF Nitrocellulose NitrocelluloseSuperSignal West Pico PLUS substrate outperforms Bio-Rad Clarity substrate. Detection of the indicated targets was performed using 2-fold serial dilutions of HEK293 or HeLa cell lysates. Chemiluminescent detection of indicated target and substrate comparison was performed, following a 5 min incubation with either SuperSignal West Pico PLUS or Bio-Rad Clarity substrate.Learn more at thermofi sher.com/chemisubstrates42'