b'SeparateTransferDetectLow-abundance target or weakly immunoreactive antigens? When a protein or antigen is difficult to detect becauseWithout enhancer SuperSignal Western Blot Enhancerof low abundance or poor immunoreactivity, use~1 secThermo Scientifi c SuperSignal Western Blot Enhancer to signifi cantly reduce background and 3 minto enhance detection of low-abundance and weakly immunoreactive antigens. SuperSignal Western Blot Enhancer increases both signal intensity and sensitivity5 min3- to 10-fold compared to detection performed using conventional western blotting.15 minMW 1.25 2.5 5 10 MW 1.25 2.5 5 10Load (g) Load (g)SuperSignal Western Blot Enhancer reduces background to enable detection of low-abundance targets.Optimize further: reprobe your western blotsReprobing a western blot saves time and conservesalternate conditions or with another antibody to detect sample while allowing optimization to be performed asa diff erent protein target. Thermo Scientifi c Restore needed. These specially formulated buff ers are designed tostripping buff ers safely and eff ectively remove primary dissociate and strip primary and secondary antibodies fromand secondary antibodies so blots can be reprobed. western blots so that membranes can be reprobed under Restore Fluorescent Western Restore Stripping Bu er Restore Plus Stripping Bu er Blot Stripping Bu erSelect when Primary antibody is susceptible toRemoving high-affinityPerforming fl uorescent stripping buff ers primary antibodies western blotsMembrane Nitrocellulose and PVDF Nitrocellulose and PVDF Use with low-fl uorescence PVDF membranes (e.g., Cat. No. 22860)Time of incubation 1530 min at 37C 515 min at RT or 37C for1020 min at RThigh-affinity antibodies45'