b'SeparateTransferDetectChoose the right gel percentageBolt NuPAGE Novex NuPAGE NovexIn general, the size of the molecule being separated shouldBis-Tris Plus Bis-Tris Tris-Glycine Tris-Acetate Tricinedictate the acrylamide or agarose percentage you choose. Use a lower percentage gel to resolve larger molecules and a higher percentage gel to resolve smaller ones. Refer to the gel migration chart (right) to find the gel best suited for your application. As a general rule, molecules should migrate through about 70% of the length of the gel for the best resolution. When protein molecular weights are wide-ranging or unknown, gradient gels are usually the best choice.Improve weak or faint bands Bolt Bis-Tris Plus and Novex Tris-glycine mini gels feature a high-capacity WedgeWell design that accommodates % of length of gelmore sample volume compared to traditional wells. The larger well capacity allows detection of proteins in very dilute samples or visualization of low-abundance proteins. Standard tips can be used when loading.Maximum loading volumes per well Maximum loading volumeComb type 1.0 mm thickness10-well WedgeWell 60 L12-well WedgeWell 45 L15-well WedgeWell 35 L17-well WedgeWell 30 L10-well25 L12-well 20 L15-well 15 L17-well 15 L12+2 well* 45 L (12 wells), 15 L (2 wells)20-well* 25 L26-well* 15 L* Midi gel.Find out more at thermofisher.com/proteingels38'