b'SeparateTransferDetectAvoid poor separation and poor band resolution Get optimal separation with the right gel for your target proteinThe choice of whether to use one chemistry or anotherProtein Gel Welcome Packs are available for each of our protein gels. Each welcome pack provides all of the necessary gels, buff ers, and depends on the abundance of the protein yourereagents you need to get started, as well as the Mini Gel Tank.separating, the size of the protein, and your downstreamFind out more at thermofi sher.com/proteingelwelcomeapplication. For separation of a broad range of proteins, two chemistriesBis-Tris and Tris-glycineare well suited. Bis-Tris gel chemistry provides greater sensitivity for proteinof protein or when your downstream applications require detection compared to Tris-glycine gel chemistry. Choosehigh protein integrity, such as posttranslational modifi cation Bis-Tris gel chemistry when you have a low abundanceanalysis, mass spectrometry, or sequencing.Bis-Tris Tris-glycine Tris-acetate TricineProtein sampleBroad range MW (6400 kDa) Broad range MWHigh range MWLow range MW type (6400 kDa) (40500 kDa) (2.540 kDa)ChemistryNeutral pH for high-sensitivityTransfer and analysis ofTransfer and analysis benefi tsapplications and reducedTraditional Laemmli-style high molecular weightof low molecular protein degradation proteins; neutral pH weight proteinsWestern blotting,Western blotting, in- High molecular weight mass spectrometry,gel staining, samplesproteins, western blotting,Low molecular weight Recommendedposttranslationally modifi edcontaining detergentsmass spectrometry,proteins, western forproteins, dilute samples, andand high salt, nativeposttranslationallyblotting, in-gel staininglow-abundanceproteins PAGE applications modifi ed proteins, native PAGE applicationsFind the right buff ers for the best separationGel chemistry Product Running buff er Transfer buff erSmall- to medium-sized proteins: Bis-Tris Bolt Bis-Tris Plus gelsMES SDS buff er Bolt or NuPAGE transfer buff erNuPAGE Bis-Tris gels Medium- to large-sized proteins: MOPS SDS Buff erTris-glycine Novex Tris-glycine gels Denaturing: Tris-glycine SDS buff erNovex Tris-glycine transfer buff erNative: Tris-glycine native buff erTris-acetate NuPAGE Tris-acetate gels Denaturing: Tris-acetate SDS buff er NuPAGE transfer buff erNative: Tris-glycine native buff erTricine Novex Tricine gels Tricine SDS buff er Novex Tris-glycine transfer buff erFind your protein gel using the Protein Gel Selection Guide at thermofi sher.com/proteingels37'